Optimization of ergosterol extraction from Pleurotus mushrooms using response surface methodology (RSM)
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resumo
Pleurotus genus represents one of the most widely consumed mushrooms in the world with interesting healthpromoting
benefits, mainly due to their richness in several bioactive compounds [1]. Mushrooms produce
ergosterol as one of their main sterols, which has been considered a contributor to their anti-inflammatory,
antioxidant, and antitumor properties [2]. Obtaining an ergosterol enriched extract depends on different
variables, such as the extraction method, solvent type, temperature, extraction time, and the solid-liquid ratio [2].
Therefore, it is essential to define the main variables and relevant response criteria to maximize the extraction
yield and purity, taking into account cost functions.
In the present work, response surface methodology (RSM) was applied to optimize a heat assisted extraction
system (HAE), combining time (t) and temperature (T) effects, and using a circumscribed central composite
design (CCCD) for the recovery of ergosterol from the fruiting bodies of P. ostreatus and P. eryngii produced
with lignocellulose substrate. Both mushrooms (POG and PEG for P. ostreatus and P. eryngii) and their byproduct
(POR and PER for P. ostreatus and P. eryngii) were used in the present work. The obtained responses
were the quantification of ergosterol by HPLC-UV (Y1: mg of ergosterol per g of extract residue and Y2: mg of
ergosterol per 100 g of dry weight mushroom).
