Ergosterol rich-extracts from Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm: A comparative study between mushroom and its bio-residues
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resumo
Edible, medicinal, and wild mushrooms are the three major components of the global mushroom industry, recently accounted for US$ 38.13 billion, and expanding at a compound annual growth rate (CAGR) of 7.9% from 2018 to 2026 [1]. Depending on the mushroom
industry size, a large amount of bio-residues is generated and often discarded (20 to 35% in weight of fresh mushrooms), even though
their content in biomolecules is not necessarily compromised [2].
Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm is one of the most produced edible mushrooms worldwide due to its ability to colonize and
degrade a large variety of lignocellulosic substrates [3]. In the present work, P. ostreatus bio-residues (POR) and intact mushrooms (POG)
were compared for their ergosterol content. Response Surface Methodology (RSM) was applied using heat-assisted extraction
methodology. The combined effect of time (10-150 min) and temperature (30-90°C) was performed using a circumscribed central
composite design (CCCD), and the response criteria determined using the HPLC-UV were ergosterol content in mg/g (ergosterol purity)
and mg/100g dw (ergosterol extraction yield)