Histological lesions in hpv16-transgenic model: the effect of hidroethanolic extract of Lavandula pedunculata (mill.) Cav.
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The K14HPV16 mice is a skin squamous carcinoma model that can be
used to test antitumoral properties of several chemical and natural
compounds1. Lavandula penduculata (Mill.) Cav., known as lavender,
belongs to the Laminaceae family and has been used in traditional
medicine as infusions to treat several conditions2. This work aimed to
evaluate the effects of the hydroethanolic French lavender extract (FLE) in
an HPV16-transgenic mice model lesions.
The extract was obtained through a maceration with ethanol/water
(80:20, v/v) and its phenolic composition was determined by HPLC-DADESI/
MS. The FLE was dissolved in drinking water at 6.8 mg/10mL/animal
and the animals were supplemented during 29 consecutive days. Twentyeight
male mice were randomly divided into four groups: (n=7/group):
group I (HPV16- control); II (HPV16- FLE); III (HPV16+ control) and IV
(HPV16+ FLE). After 29 days all animals were sacrificed by xylazineketamine
overdose following cardiac puncture to obtain blood samples.
Skin samples (chest and ear), kidney, liver and spleen were processed for
histological analysis.A total of thirteen compounds were identified in the hydroethanolic
extract, being salvianolic acid B and rosmarinic acid the main molecules
present. Moreover, the compounds revealed to be stable in the drinking
water for 5 days. Histological analyses of skin samples from wild-type
mice exposed (group II) and not exposed (group I) to FLE showed normal
skin histology. Group III showed skin chest epidermal hyperplasia in 100%
of the mice while group IV showed less epidermal hyperplasia frequency
(66.6%) (p>0.05). Concerning to liver, kidney, and spleen lesions there are
no differences between groups (p>0.05). The lavender extract did not
prevent the progression of HPV-16 induced cutaneous lesions in this
model. These data deserve more investigation to clarify the effect of
lavender extract on HPV-16 lesions.
