Antioxidant properties of different dietary supplements based on Agaricus blazei Murrill
Artigo de Conferência
Visão geral
Visão geral
resumo
In 2012, the market for nutraceuticals earned $90 billion worldwide and it is predicted to reach $33.6
billion in the next four years. Agaricus blazei Murrill mushroom is native to Brazil and widely
cultivated due to nutritional and medicinal value [I ,2]. Its beneficial effects have long been
recognized, becoming popular as ordinary food, increasing also its production and marketing as
dietary supplements (3].
A. blazei is traditionally used to combat a variety of diseases such as cancer, diabetes, hepatitis and
hypercholesterolemia [2]. The use of dietary supplements based on A. blazei has grown significantly,
due to pharmacological studies reporting antioxidant, antitumor, anti-inflammatory, antimicrobial,
immunostimulant, apoptotic and chemopreventive effects [2,3], attributed to ®-glucans and other
bioactive compounds, such as steroids, tocopherols and phenolic compounds [4]. Herein, the
antioxidant activity of A. blazei capsules enriched with fmits of Malpighia glabra L. (acerola),
Solanum melongena L. (eggplant) and Euterpe oleracea Mart. (açai), calcium and chitosan, were evaluated though in vitro assays: DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity,
reducing power, inhibition of G-carotene bleaching and inhibition of lipid peroxidation in brain
homogenates by thiobarbituric acid reactive substances (TSARS) assay. Two capsules (daily
recommended dose) were dissolved in 100 mL of distilled water in order to prepare a stock solution.
Several dilutions were tested until determination of EC50 values (concentration responsible for 50% of antioxidant activity or 0.5 of absorbance in reducing power assay).
Globally, all the tested formulations showed high antioxidant activity. A. blazei with acerola gave the
highest DPPH scavenging activity (EC50=0.81±0.0 1 mg/mL) and reducing power (EC50=0.53±0.0 1 mg/mL). Nevertheless, A. blazei with ayai showed the highest activity in the lipophilic assays: f3 -
carotene bleaching inhibition (EC50=0.55±0.02 mg/mL) and lipid peroxidation inhibition by TSARS
assay (EC50=O. 14±0.01 mg/mL). The studied formulations might be useful as antioxidants-enriched supplements to prevent some of the diseases related to oxidative stress.
