The phenolic profile of barley (Hordeum vulgare L.) leaves, seeds, awns, and stems, collected in two
different locations from Portugal, was determined by a high-performance liquid chromatography/
diode array detector (HPLC/DAD). A total of 28 compounds were identified and quantified, which
included 4 phenolic acids, 6 C-glycosylflavones, and 18 O-glycosyl-C-glycosyl flavones, with some
of them acylated. Distinct profiles were noticed among the analyzed materials. The greatest diversity
of compounds was found in barley leaves (26 flavonoids and 2 phenolic acid derivatives), which also
exhibited the highest concentration of phenolics. Isoorientin-7-O-glucoside (lutonarin) was the major
compound in leaves, while, in general, the pair isovitexin-7-O-rutinoside plus isoscoparin-7-O-glucoside
were the main phenolics in the other materials. Thus, barley leaves may constitute an important
dietary source of protective compounds, which could be used, for example, to take profit from the
wastes resulting from alcoholic drink obtainment.
