Search for microorganisms in antiseptic, disinfectant and detergent dispensers of a Local Health Care Unit from Northeast Portugal
Artigo de Conferência
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resumo
Introduction: Health care associated infections (HCAI) are among the main causes of
mortality worldwide, and of the increase of internment time and related costs. Many
HCAI acquired in hospitals result from improper disinfection practices of medical
devices and surfaces of the hospital environment, as also insufficient asepsis of health
professional’s skin [1,2].
Objectives: The main objectives of the present work were to verify the presence of
microorganisms in antiseptic, disinfectant and detergent dispensers from different
services of Bragança’s Local Health Care Unit, and to determine their resistance
profiles.
Materials and Methods: 151 Samples were collected from antiseptic (alcoholic
solution, povidone-iodine and chlorhexidine), disinfectant (70% alcohol) and detergent
(dermatological soap) dispensers, and futher plated in Columbia CNA + 5% sheep
blood and MacConkey agar. The identification and the bacterial antibiogram were
performed using Vitek 2.
Results and Discussion: Among the 151 samples plated, 25 exhibited
contamination, corresponding to a contamination percentage of 17%. The most
frequently identified and isolated microorganisms were Staphylococcus hominis, Sphingomonas paucimobilis and Staphylococcus epidermidis with a percentage of
20%, 20% and 16%, respectively. Micrococcus luteus/lylae, Rhizobium radiobacter,
Staphylococcus capitis, Pseudomonas luteola, Staphylococcus warneri,
Staphylococcus vitulinus, Kocuria kristinae and other gram-positive bacillus were
identified with less prevalence. The dermatological soap was the product with the
highest contamination percentage and it was in the Medicine services that more
contaminated products were found, while Surgery services showed the lowest
contamination. Some of the isolated bacteria showed important resistance profiles,
such as Staphylococcus hominis and Staphylococcus epidermidis, namely to betalactams
(oxacillin and amoxicillin/clavulanic acid) and quinolones (levofloxacin).
Conclusion: Although the main isolated microorganisms belong to a group of
commensal bacteria that do not cause problems to a person with a normal immunity
system, these are responsible for HCAI. Therefore, it is essential to develop protocols
for handling and packaging all the solutions in order to decrease the contamination
level and, consequently, the HCAI.
