Plant extract with bioactive potential against SARS-CoV-2
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The novel disease caused by SARS·CoV-2 has been causing chaos worldwide due to its
high mortality, morbidity and contagiousness nature, and therefore forced the scientific world to
develop n'ew alternatives to combat and assist the fight against this virus. Plant extracts have bioactive
molecules that might provide a starting point for the research on the use of plants as an excellent
source ofoew antiviral agents able to inhibit or neutralize COVID-19. Therefore, the objective ofthis
study was to obtain an extract !Tom a Plaot cultivated under controlled conditions in Portugal to be
used as ao antiviral substance to be applied against COVID-19.
MaterialslMethods: The plant extract obtained !Tom plant cultivated under controlled conditions in
Portugal was extracted by maceration in a hydroethanolic solution under optimized conditions and
submitted to freeze drying. The extracts were evaluated for their phenolic profile by high performance
liquid chromatography coupled to a diode array detector and a mass spectrometer detector (HPLCDAD-
MS). Antioxidant activity was also measured, and antiviral properties were measured for SARSCoV-
2 in Vero cells.
Remlts. HPLC analysis revealed the presence of different phenolic compounds in the extracts with
relevance for caftaric acid, cis-chicoric acid and trans-chychoric acid. The extract showed a good
antioxidant capacity demonstrated by the high value found for aRAC of3202,82 ± 32,52 Troloxlmg
extrac!. Regarding the antiviral activity, the results achieved for the viral titer was 20 PFU/mL, and the
antiviral activity for SARS-CoV-2 was 4.02 ± 0.00 Mv and the perceotage ofreduction was > 99.99%.
Conclusion: The results showed that the obtained extract demonstrated consistent results of antiviral
activity presenting a potential for applications against the SARS-CoV-2. Further studies are required
for validation and application of this extract.
