Evaluation of minimum inhibition concentrations of plant against environmental fungi and dermatophytes Artigo de Conferência uri icon

resumo

  • The outbreak of COVID-19 disease eaused by SARS-CoV-2 has led the seientifie eompmnity to seareh for new alternatives to help eontrol the virus. In this context, the use of face masks has been reeommended as a preventive measure against the spread of SARS-CoV-2. The application of antimierobial agents in the tissues used to produee masks would be an additional hurdle on the prevention of other respiratory infeetions and seeondary fungaI infeetions that oeeur from touehing eontaminated masks. In this eontext, impregnation of the fabries with plant derived extraets is an attraetive approaeh sinee they are potential1y safe, free of adverse side effeets, and powerful antimierobials. The objeetive of this work was to study fifteen plant extraets to seleet the most effieient against environmental fungi and some dermatophytes. MaterialslMethods: The minimum inhibitory eoneentration (MIC) of eaeh plant derived extraet was determined by broth mierodilution in 96-wells mierotiter plates. Coneentrations ranging from 50 mg/mL to 1.5625 mg/mL were tested against lhe different fungi. Briefly, 100 mL of a 105 spores/mL solution were added to 100 mL of eaeh extraet eoneentration. Afier 48 h, 10 mL of eaeh well were plated onto Sabouraud Dextrose Agar. Cultivation was earried out at 25 °C for 3 days for the environmental fungi and 7 days for the dermatophytes. The MICs were regarded as the lowest coneentrations that did not allow any visible growth when eompared with the eontrol sets. Results: The results for the fifteen plant extraets tested against nine environmental fungai species revealed that only one extraet was able to inbibit fungai growth of two of (hese fungi (Cladosporium sp. and F. verticillioides) at a eoneentration of 50 mg/mL. The results against the two filamentous fungi dermatophytes (T. mentagrophytes and M canis) revealed these fungi to be the lllf'st susceptible to the extraets tested. Most of the extraels inhibited dermatophyte growth at eoneettrations < 50 mg/mL with the lowest MIC being registered at 3.125 mg/mL. Results for the yeast M. furfur revealed that only three plant derived extraets inhibited its growth with eoncentrations between 30 and 50 mg/mL. Conclusion: The plant extraets tested against environmental fungi and dermatophytes revealed to be more efficient against filamentous fungi dermatophyte growth. Environmental fungi followed by lhe dermatophyte yeast, showed the highest resislanee to these pIant extracts.

data de publicação

  • novembro 2021